Fusion and development of 2-cell bovine embryos to tetraploid blastocyst with different voltages and durations

Abstract Background: The values of embryonic stem cell and cloning are evident. Production of clone from embryonic stem cells can be achieved by introduction of stem cell into a tetraploid blastocyst. Tetraploid blastocyst can be produced in vitro by electrofusion of 2-cell embryos. Objective: The aim of this study was to assess the effect of different voltages and durations on fusion rate of bovine 2-cell embryos and their subsequent development in vitro. Material and Methods: The in vitro produced bovine 2-cell embryos were categorized into 3 groups: (1) fused group (FG); 2-cell embryos fused by exposure to different voltages (0.5, 0.75, 1, 1.25 and 1.5 kV/cm) and durations (20, 40, 60, 80 and 100 μs), (2) exposed control group (ECG); 2-cell embryos exposed to different voltages and durations but remained unfused and (3) unexposed control group (UCG); embryos cultured without exposure to any voltage. The embryos from each group were cultured and fusion, cleavage and developmental rates were compared in each group. Results: The results show that increased voltage, increases the fusion rate up to 88% for 1.5 kV/cm; however, the rate of cleavage and blastocyst formation decreases significantly to 18% and 10% respectively (p<0.05). Increased duration does not significantly increase fusion rate, however, in high voltage, increased duration decreases cleavage rate and blastocyst formation rate. Blastocyst formation rate in UCG showed a better development (32%) compared to FG (20%) or ECG (22.5%) (p<0.05). Conclusion: It can be concluded that for optimal fusion, cleavage and development, one pulse of 0.75 kV/cm for 60μs should be applied.

Association of Sterile Pyuria and Coronary Artery Aneurysm in Kawasaki Syndrome

Abstract- Kawasaki disease (KD) is an inflammatory multiorgan disease of unknown etiology. The most dramatic organ involved is the heart. There were a few studies about cardiac involvement and sterile pyuria. This study guides to determine if sterile pyuria is associated with coronary artery aneurysm (CAA) in KD patients and to consider it as a predicting factor for coronary artery involvement. Forty seven patients with KD were studied by echocardiography in admission and one month later. Urine analysis, complete blood count, erythrocyte sedimentation rate and C-reactive protein were measured in admission. Data were analyzed using SPSS-14 software. Patients' age was ranged from 13 month to 7 years old (mean age of 3.43±1.54 years). Thirty patients (63.8%) were male and 17 patients (36.1%) were female. Cardiac involvement was detected in 32 patients (68%) using echocardiography, of which CAA was reported in 8 cases (17%). Six of CAA (75%) were in association with sterile pyuria, although it was statistically insignificant (P>0.05). Although the majority of patients with CAA had sterile pyuria, this association is not statistically significant, thus it couldn't be considered as a predicting factor for CAA.

Lunching the Internet Reception of patients Imam Reza Specialized and Subspecialized plyclinic

Increase 500 beds to Vali Asr Hospital beds

Governor General of Markazi province said that an increase of 500 beds to the total number of Vali Asr hospital beds is in the follow-up.

Non-communicable diseases are the main health threat in the Markazi province

Health deputy of Arak University of Medical Sciences said that non-communicable diseases are the most important threat to the health of the people of the province, which will be controllable according to prevention and care plans

Establishment of Markazi province food security document is on the agenda

Political, Security and Social affairs deputy of governor general of Markazi province said that establishment of Markazi province food security document is on the agenda

The motto of the 2018 Health Week was announced.

The secretary general of the Health Week in Markazi province described the slogan of Health Week in Markazi province

The need for serious attention to health in policy making of executive devices

Vice-chancellor for Social affairs of Minister of Health and Medical Education in Arak said that the executive devices should adopt policies that, while developing and progressing the country, will also pay close attention to the health issue.

Strengthening Health sector, with public participation is possible

Representative of Arak , Komijan and Khondab in Parliament said that strengthening the Health sector with its socialization and attracting public participation is possible and the formation of Health assembly is an attempt in this direction.